This book shows how these techniques provide useful molar mass, architectural, compositional, and thermodynamic information whether SEC is used as the sole separation method or as part of a two-dimensional chromatographic set-up.
In the latter case, the ability to separate analytes based on size, provided by SEC, is augmented by separation based on chemical composition, block length, etc., provided by high performance liquid chromatography, liquid chromatography at the critical condition, phase fluctuation chromatography, or high osmotic pressure chromatography.
This is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC).
SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution.
The Handbook contains contributions from every column manufacturer around the world and from many experienced column users.
It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC.
Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner.
This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC).
The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography.
Multiple Detection in Size-Exclusion Chromatography provides a comprehensive view of detection techniques that are used synergistically in size-exclusion chromatography (SEC).
These techniques include differential refractometry; static and dynamic light scattering; differential viscometry; fluorescence, ultraviolet, infrared, and nuclear magnetic resonance spectroscopy; electrospray ionization, matrix-assisted laser desorption ionization, chemical reaction interface, inductively coupled plasma, and tandem mass spectrometry; and dynamic surface tension.
Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.
This book covers recent advances in hyphenated and multidimensional chromatographic techniques for elucidating the structure, composition, molecular weight, and branching distributions in complex polymers.