The aim of this study is to find out whether expression of these proteins is regulated in parallel or independently and to compare the expression profiles of are encoded by at least two to four genes, which might be indicative for the expected variability and presence of isoforms.
You’ll still be able to search, browse and read our articles, but you won’t be able to register, edit your account, purchase content, or activate tokens or eprints during that period.The low-temperature stress was applied by transferring the plantlets to a cold room at 4 °C in the dark.For high temperature stress, the plantlets were exposed to 37 °C in the dark.The sterile 5 m M salicylic acid solution was sprayed onto PIS plantlets at room temperature.For wounding stress, two leaves (one at the top and one at the bottom) of each plantlet were wounded with a needle, 3–4 times along the blade.Considering the important role of Ca M and LTP in mediating plant stress responses, and based on the shortage of data in woody plants, species to environmental stresses.Embryogenic calli of PIS were induced on isolated roots of in vitro plantlets following the protocol of Druart and De Wulf (1993).Primers Ca M-Real-Left and Ca M-Real-Right (5′-TCCGGGTGTTTGATAAGGAC-3′ and 5′-CAGCCTCACGAATCATCTCA-3′) amplifying a 120 bp fragment were designed specific to the sp. BQ641140, X96714, AY792996, AY620230 and AF221501). The Real Time PCR was carried out in the Rotor-Gene 2000 (Corbett Research) with a 72-well rotor.The amplification reactions were performed in 16 μl final volume containing 8 μl of a 2 × Power SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK), 0.96 μl of primer-pair mix (primer concentration: 300/300 n M) and 2 μl c DNA first-strand template.Western blot analyses indicated that after 24 h the amount of Ca M protein is higher, while the amount of LTP is lower in various stresses. The Ca M isoforms may activate selected subsets of target proteins and be induced by appropriate stimuli or physiological stresses in different tissues.Results obtained suggest that Ca M and LTP are differentially regulated in response to different stresses in PIS plants, and additionally show tissue-specific expression, hinting at a potential role of different isoforms. Calmodulin (Ca M), an ubiquitous Ca-dependent cellular processes and target molecules involved in plant stress response. The PR proteins are classified into 17 families based on sequence, structure, function and biological activity (van Loon and van Strien 1999).